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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was observed to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential part in advertising cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't yet offered.
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Blocking peptides are peptides that bind specially on the goal antibody and block antibody binding. These peptide typically consists of the epitope regarded because of the antibody.
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This tutorial outlines more doable optimization of cellular and immunoassay parameters to make sure the best possible success are...
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This tutorial outlines further more attainable optimization of mobile and immunoassay parameters to make certain the best possible benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the target protein. By comparing the staining from the blocked antibody vs . the antibody alone, one can see which staining is precise.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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